Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues

Frustrated FRET for high-contrast high-resolution two-photon imaging.

Two-photon fluorescence microscopy has become increasingly popular in biomedical research as it allows high-resolution imaging of thick biological specimen with superior contrast and penetration than confocal microscopy. However, two-photon microscopy still faces two fundamental limitations: 1) image-contrast deterioration with imaging depth due to out-of-focus background and 2) diffraction-lim...

High-Contrast Imaging and High-Resolution Spectroscopy Observation of Exoplanets

Detection and characterization of exoplanets faces challenges of smaller angular separation and high contrast between exoplanets and their host stars. High contrast imaging (HCI) instruments equipped with coronagraphs are built to meet these challenges, providing a way of spatially suppressing and separating stellar flux from that of a planet. Another way of separating stellar flux can be achie...

High Resolution and High Sensitivity Imaging of Charge Distribution

Physical characterization of semiconductor device has always been a challenging task for device engineers and researchers. Up to now, standard methods for characterizing semiconductors did not provide an effective means for determining two-dimensional quantities of sub-device scale. These methods include Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Secondary Ion M...

Photoacoustic contrast imaging of biological tissues with nanodiamonds fabricated for high near-infrared absorbance.

Radiation-damaged nanodiamonds (DNDs) are potentially ideal optical contrast agents for photoacoustic (PA) imaging in biological tissues due to their low toxicity and high optical absorbance. PA imaging contrast agents have been limited to quantum dots and gold particles, since most existing carbon-based nanoparticles, including fluorescent nanodiamonds, do not have sufficient optical absorptio...

High resolution magnetic imaging: MicroSQUID Force Microscopy